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anti ccl22 mdc antibody  (R&D Systems)


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    R&D Systems anti ccl22 mdc antibody
    Anti Ccl22 Mdc Antibody, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+ccl22/Human+CCL22%2FMDC+Antibody/pm42003899-49-1-9
    Average 93 stars, based on 11 article reviews
    anti ccl22 mdc antibody - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Incubation:

    Article Title: CCL22-induced responses are powerfully enhanced by synergy inducing chemokines via CCR4: evidence for the involvement of first beta-strand of chemokine.
    Article Snippet: Chemokine solutions containing CCL22, CCL19 or CCL22 plus CCL19 (500 ng), were incubated at 4 C overnight with monoclonal antibodies (1 lg) to CCL22 or CCL19 (MAB336 and MAB361, R&D, Minneapolis, MN, respectively). .. Solutions were incubated with biotinylated rabbit anti-mouse antibody (8 lg, E0413, DAKO, Germany) for an additional 2 h. Immune complexes were isolated with 25 ll streptavidin Dynabeads (M-280 Dynal, Norway) and samples analyzed by Western blotting on polyvinylidene difluoride (PVDF) membranes using 0.2 lg/ml anti-CCL22 (polyclonal chicken anti-human AF 336, R&D) or 2 lg/ml anti-CCL19 (MAB361, R&D). .. Enhanced chemiluminescence was used for detection of HRP-conjugated secondary antibodies (703-035-155 Jackson ImmunoResearch labs for CCL22, and 170-6516, Bio-Rad for CCL19, respectively) following the manufacturer's instructions (34080 West Pico Chemiluminescent Substrate, Pierce, Rockford, IL).

    Isolation:

    Article Title: CCL22-induced responses are powerfully enhanced by synergy inducing chemokines via CCR4: evidence for the involvement of first beta-strand of chemokine.
    Article Snippet: Chemokine solutions containing CCL22, CCL19 or CCL22 plus CCL19 (500 ng), were incubated at 4 C overnight with monoclonal antibodies (1 lg) to CCL22 or CCL19 (MAB336 and MAB361, R&D, Minneapolis, MN, respectively). .. Solutions were incubated with biotinylated rabbit anti-mouse antibody (8 lg, E0413, DAKO, Germany) for an additional 2 h. Immune complexes were isolated with 25 ll streptavidin Dynabeads (M-280 Dynal, Norway) and samples analyzed by Western blotting on polyvinylidene difluoride (PVDF) membranes using 0.2 lg/ml anti-CCL22 (polyclonal chicken anti-human AF 336, R&D) or 2 lg/ml anti-CCL19 (MAB361, R&D). .. Enhanced chemiluminescence was used for detection of HRP-conjugated secondary antibodies (703-035-155 Jackson ImmunoResearch labs for CCL22, and 170-6516, Bio-Rad for CCL19, respectively) following the manufacturer's instructions (34080 West Pico Chemiluminescent Substrate, Pierce, Rockford, IL).

    Western Blot:

    Article Title: CCL22-induced responses are powerfully enhanced by synergy inducing chemokines via CCR4: evidence for the involvement of first beta-strand of chemokine.
    Article Snippet: Chemokine solutions containing CCL22, CCL19 or CCL22 plus CCL19 (500 ng), were incubated at 4 C overnight with monoclonal antibodies (1 lg) to CCL22 or CCL19 (MAB336 and MAB361, R&D, Minneapolis, MN, respectively). .. Solutions were incubated with biotinylated rabbit anti-mouse antibody (8 lg, E0413, DAKO, Germany) for an additional 2 h. Immune complexes were isolated with 25 ll streptavidin Dynabeads (M-280 Dynal, Norway) and samples analyzed by Western blotting on polyvinylidene difluoride (PVDF) membranes using 0.2 lg/ml anti-CCL22 (polyclonal chicken anti-human AF 336, R&D) or 2 lg/ml anti-CCL19 (MAB361, R&D). .. Enhanced chemiluminescence was used for detection of HRP-conjugated secondary antibodies (703-035-155 Jackson ImmunoResearch labs for CCL22, and 170-6516, Bio-Rad for CCL19, respectively) following the manufacturer's instructions (34080 West Pico Chemiluminescent Substrate, Pierce, Rockford, IL).



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    a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, <t>CCL22</t> and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.
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    Image Search Results


    a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, CCL22 and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.

    Journal: Experimental & Molecular Medicine

    Article Title: H3K18 lactylation in cancer-associated fibroblasts drives malignant pleural effusion progression via TNFR2 + T reg recruitment

    doi: 10.1038/s12276-025-01557-3

    Figure Lengend Snippet: a KEGG pathway enrichment of DEGs between FACS-sorted TNFR2 + and TNFR2 − CD4 + T cells from MPE ( n = 3). b , c Volcano plot ( b ) and heatmap ( c ) of DEGs related to the chemokine signaling pathway. d , e Flow cytometry histograms ( d ) and comparisons of chemokine receptor expression (CXCR6, CCR4 and CCR6) ( e ) on TNFR2 + T reg cells and TNFR2 − T reg cells ( n = 13). f Schematic of Transwell chemotaxis assay testing TNFR2 − T reg chemotaxis toward MPE supernatant (by Figdraw). g , h Transwell chemotaxis assay comparing TNFR2 + T reg frequencies between freshly isolated cells and cells that migrated toward MPE supernatant after 4 h incubation. i Concentrations of CXCL16, CCL17, CCL22 and CCL20 in MPE and PB were quantified using ELISA ( n = 16). j Schematic of chemotaxis assay to investigate the chemotatic axis to attract TNFR2 + T reg cells in MPE. k , l Flow cytometry histograms and comparisons of chemotaxis of TNFR2 + T reg cells in response to MPE in the presence of anti-CXCL16, anti-CCL17, anti-CCL22 or anti-CCL20 mAbs. Data shown in d , e , g – i , k and l are representative of at least three independent experiments (mean ± s.d.). Statistical analysis was performed using paired two-tailed Student’s t -test ( e and h ), Wilcoxon test ( i ) or one-way ANOVA ( l ). * P < 0.05, ** P < 0.01, **** P < 0.0001. ns not significant, mAbs monoclonal antibodies.

    Article Snippet: In specific experimental conditions, the following reagents were added to the lower chamber: anti-CCL17 antibody (50 ng/ml, #MAB364-SP, R&D Systems), anti-CCL20 antibody (50 ng/ml, #MAB360-SP, R&D Systems), anti-CCL22 antibody (50 ng/ml, #MAB336-SP, R&D Systems), anti-CXCL16 antibody (50 ng/ml, #MAB976, R&D Systems) or recombinant human CXCL16 (100 ng/ml, #976-CX-025/CF, R&D Systems).

    Techniques: Flow Cytometry, Expressing, Chemotaxis Assay, Isolation, Incubation, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Bioprocessing